differential expression of human homeodomain tgiflx in brain tumor cell lines.

glioblastoma is the most common and the most lethal primary brain cancer. this malignancy is highly locally invasive, rarely metastatic and resistant to current therapies. little is known about the distinct molecular biology of glioblastoma multiforme (gbm) in terms of initiation and progression. so far, several molecular mechanisms have been suggested to implicate in gbm development. homeodomain (hd) transcription factors play central roles in the expression of genomic information in all known eukaryotes. the tgifx homeobox gene was originally discovered in human adult testes. our previous study showed implications of tgiflx in prostate cancer and azoospermia, although the molecular mechanism by which tgiflx acts is unknown. moreover, studies reported that hd proteins are involved in normal and abnormal brain developments. we examined the expression pattern of tgiflx in different human brain tumor cell lines including u87mg, a172, daoy and 1321n1. interestingly, real time rt-pcr and western blot analysis revealed a high level of tgiflx expression in a172 cells but not in the other cell lines. we subsequently cloned the entire coding sequence of tgiflx gene into the pegfp-n1 vector, eukaryotic expression vector encoding egfp, and transfected into the u-87 mg cell line. the tgiflx-gfp expression was confirmed by real time rt-pcr and uv-microscopic analysis. upon transfection into u87 cells, fusion protein tgiflx-gfp was found to locate mainly in the nucleus. this is the first report to determine the nuclear localization of tgiflx and evaluation of its expression level between different brain tumor cell lines. our data also suggest that tgiflx gene dysregulation could be involved in the pathogenesis of some human brain tumors.